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Download Cell Cycle Checkpoints: Methods and Protocols (Methods in Molecular Biology, Vol. 782) djvu

Download Cell Cycle Checkpoints: Methods and Protocols (Methods in Molecular Biology, Vol. 782) djvu

by Willis X. Li

Author: Willis X. Li
Subcategory: Biological Sciences
Language: English
Publisher: Humana Press; 1st edition (August 30, 2011)
Pages: 307 pages
Category: Math and Science
Rating: 4.3
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Cell Cycle Checkpoints: Methods and Protocols (Methods in Molecular Biology 782).

Cell Cycle Checkpoints: Methods and Protocols (Methods in Molecular Biology 782). Willis X. Li. Download (pdf, . 6 Mb) Donate Read. Epub FB2 mobi txt RTF. Converted file can differ from the original. If possible, download the file in its original format.

Methods and Protocols. Methods for Studying the G2 DNA Damage Checkpoint in Mammalian Cells. Includes cutting-edge methods and protocols. Provides step-by-step detail essential for reproducible results.

Willis X. Li Department of Medicine, University of California, San Diego, La Jolla, CA, US. These checkpoints prevent progression into the next phase of the cell cycle until the processes at the current phase have been properly completed

Willis X. These checkpoints prevent progression into the next phase of the cell cycle until the processes at the current phase have been properly completed. The most studied cell cycle checkpoints act at transition points, such as from G1 to S, G2 to M, or the metaphase to anaphase transition. Genotoxic stresses from byproducts of cellular metabolism or environmental sources can also activate cell cycle checkpoints, halting cell cycle progression until the damages are repaired.

Methods in molecular biology; vol. 782, 2012. ing methods to study cancer therapeutic drugs. that target cell cycle checkpoints. Humana Press – Springer Verlag, Heidelberg, Germany. ISBN: 978-1-61779-272-4.

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Start by marking Methods in Molecular Biology, Volume 241: Cell Cycle Checkpoint Control Protocols as Want to Read: Want to Read savin. ant to Read. Using mammalian, yeast, and frog systems, these readily reproducible methods can be used to induce cell cycle checkpoints, detect changes in cell cycle progression, identify and analyze genes and proteins that regulate the process, and A collection of basic cutting-edge techniques for studying the mechanisms underlying cell cycle regulation and checkpoint control.

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Series: Methods in Molecular Biology 782.

Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Series: Methods in Molecular Biology 782.

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Cell cycle checkpoints control the fidelity and orderly progression of eukaryotic cell division. By controlling the orderly progression of critical cell cycle events such as DNA replication and chromosome segregation and ensuring proper repair of damaged DNA, cell cycle checkpoints function to ensure genome integrity. Mechanisms of checkpoint controls are not only the research focus of investigators interested in mechanisms that regulate the cell cycle, but are also the interests of researchers studying cancer development as it is increasingly clear that loss of cell cycle checkpoints, which leads to genomic instability as a result, is a hallmark of tumorigenesis. Cell Cycle Checkpoints: Methods and Protocols provides detailed descriptions of methodologies currently employed by researchers in the field, including those commonly used in the mammalian, yeast, C. elegans, Drosophila, and Xenopus model systems. Each chapter describes a specific technique or protocol, such as a method to induce cell cycle checkpoints in a particular model system, to synchronize a population of cells to allow observations of cell cycle progression, to identify genes involved in checkpoint regulation, and to study particular protein components of cell cycle checkpoint pathways. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, Cell Cycle Checkpoints: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies in an effort to further our knowledge of this essential field.